Journal: International Journal of Molecular Sciences

Article Title: Dietary Intake of 17α-Ethinylestradiol Promotes HCC Progression in Humanized Male Mice Expressing Sex Hormone-Binding Globulin

doi: 10.3390/ijms222212557

Figure Lengend Snippet: Pro-inflammatory response and compensatory proliferation were promoted in SHBG mice fed with EE2. ( A ) Western blot analysis and quantification of intact and phosphorylated p65 NF-κB and IκBα in livers of WT EE2 and SHBG EE2 mice. β-Actin was used for an internal control. ( B ) mRNA expression of IL-1β , Tnf, Ccl2 in livers of WT EE2 and SHBG EE2 mice. Rplp0 was used for an internal control. ( C ) Western blot analysis and quantification of intact and phosphorylated EGFR, and PCNA in livers of WT EE2 and SHBG EE2 mice. β-Actin was used for an internal control. ( D ) mRNA expression of Ccrk , Cyclin D , Ki67 , C-Fos , and C-Jun in livers of WT EE2 and SHBG EE2 mice. Rplp0 was used for an internal control. Numbers of mice used for experiments were: WT EE2 (8), SHBG EE2 (8). Student’s t -Test was used for analysis. Values represent means ± SD. * p < 0.05. Data were quantified from replicated values in which independent experiments were performed in triplicate at least.

Article Snippet: Primary antibodies for the following proteins were used: PARP (9930T, Cell signalling Technology; CST, Danvers, MA, USA), cleaved PARP (9930T, CST), SHBG (sc-377032, Santa Cruz, Dallas, TX, USA), AR (5133, CST), β-actin (sc-1616, Santa Cruz), PCNA (13110, CST), EGFR (A2909, Abclonal, MA, USA), phosphor-EGFR (9789, CST), HMGB1 (CSB-PA01604A0Rb, Cusabio, Houston, TX, USA), NF-κB p65, phosphor-NF-κB p65, IκBα, and phosphor-IκBα (9936, CST).

Techniques: Western Blot, Control, Expressing

Journal: Infection and Immunity

Article Title: Thioredoxin from Brugia malayi: Defining a 16-Kilodalton Class of Thioredoxins from Nematodes

doi: 10.1128/IAI.71.7.4119-4126.2003

Figure Lengend Snippet: Bm-TRX-1 inhibits activation of p38 MAP kinase. The WCPPCR and WCPQCR alleles of Bm-trx-1 were cloned separately into a eukaryotic expression plasmid vector, and the plasmids were introduced into TPA-differentiated ML-1 cells. The transfected ML-1 cells were stimulated with TNF-α (100 IU/well) for 5 min, and the phosphorylation status of p38 MAP kinase was analyzed on immunostained Western blots of cell extracts. Controls included cells maintained in medium (medium control), cells exposed to the transfection regent (transfection control), and cells transfected with a plasmid containing no insert (plasmid control).

Article Snippet: In preliminary experiments, TPA-differentiated ML-1 cells were stimulated with TNF-α (100 IU/well) for 5, 10, and 30 min, and the phosphorylation status of p38 MAP kinase was analyzed on immunostained Western blots of cell extracts with phosphoPlus p38 MAP kinase antibody kit (Cell Signaling Technology, Beverly, Mass.).

Techniques: Activation Assay, Clone Assay, Expressing, Plasmid Preparation, Transfection, Western Blot